Ion Exchange Resin Column

Aside from its use to purify drinking water the technique is widely applied for purification and separation of a variety of industrially and medicinally.

Ion exchange resin column.

Common matrices are cellulose agarose polymethacrylate polystyrene and polyacrylamide. Ion exchange chromatography resin selection. Cation exchange chromatography workflow. All ion exchange chromatography relies on electrostatic interactions between the resin functional groups and proteins of interest.

Column size most industrial ion exchange columns have a diameter between 0 5 and 3 2 m. Water typically flows down through the resin bed. Ion exchange chromatography resins can be used at high flow rates because binding kinetics for iex are fast and rigid chromatography particles can be used. The latter three matrices allow higher flow rates.

Ion exchange takes place in one column while the resin in the second column is being regenerated resulting in continuous operation. For in depth information about iex download our iex handbook. Bigger vessels with up to 4 0 m are sometimes built but the aspect ratio of the resin bed width height becomes unfavourable and very precise distribution and collection systems must be fitted to ensure an even flow of the water during service and. Ion exchange usually describes a processes of purification of aqueous solutions using solid polymeric ion exchange resin more precisely the term encompasses a large variety of processes where ions are exchanged between two electrolytes.

Thus the workflow below is given as a generalized iex workflow and particular running conditions for cation exchange chromatography may be adjusted to best suit your protein of interest the buffer system and the anion. Usage examples continuous ion exchangers are used when it is imperative that product be continually generated. Rohm and haas ion exchange ion exchange introduction 4 fd sep 2008 to soften water you take a cation exchange resin on which the mobile ion inside the beads is sodium na and you pass the hard water through a column filled with the sodium form resin. While many of us have heard of ion exchange ix resins few of us have a grasp on how the technology actually works.

The hardness ions ca and mg move into the resin beads and each of these divalent. Ion exchange chromatography resins are composed of positively or negatively charged functional groups that are covalently bound to a solid matrix. Continuous ion exchange processes take place in a column or vessel containing a deep bed of ion exchange resin beads. The separation is based on the reversible interaction between a charged protein and an oppositely charged chromatography resin.

Ion exchange processes can be conducted in continuous or batch mode with most water treatment ix processes run in a continuous mode. The beads are typically porous providing a large surface area on and inside them the trapping of.